|Sampling and Holding Times
We need about 100 mls of each sample
for microbiological analyses. For anaerobes, care should be taken during sampling
so that no additional oxygen is introduced into the sample bottles. We recommend that the
bottles be filled slowly with no mixing of air, until the sample overflows the bottle.
Then cap the sample bottles, ensuring that there is no air space in the sample bottle. The
samples should be stored in a cooler (4° C) upon sampling, and delivered to us as
soon as possible (preferably within 48-72 hours, and up to 7 days for refrigerated soil/
sediment samples ). Please be sure that the samples do not freeze, and do NOT add any
For chemistry assays, pH, DO, and redox, we require One 500ml bottle (polypropylene if possible) for each sample. Samples should be stored on ice, and no
preservative should be added. pH, DO and redox measurements should be performed as
soon as possible after sampling, within 24 hours.
For inorganic chemistry assays, nitrate-N, sulfate,
ammonia-N and o-phosphate, we require one 500ml bottle for each
sample. Please ship these samples immediately to CytoCulture unpreserved,
or call for individual preservative requirements to meet longer hold times (up to 28
days). Sulfate can be held unpreserved up to 28 days.
For subcontracted biological, chemical, and gas monitoring assays,
please call for sample requirements and to have sample containers shipped to your site.
Soil and water samples are normally delivered by courier or overnight express
shipping in glass or plastic sample containers packed in blue ice or wet ice (not on dry
ice). Avoid freezing of samples or exposure to excessive heat, and be sure no
preservatives (or HCl stabilizers) have been added to the water samples.
Soil testing requires several grams of representative sample, usually
provided in 2 oz glass sample jars. Water testing requires up to one liter, in glass or
plastic containers sealed with tight screw caps. CytoCulture will hold the samples for up
to two weeks after reporting data in the event the client wished to conduct additional
tests. Please notify CytoCulture in advance, or fax a completed Chain of Custody form,
prior to delivery of samples.
The CytoCulture laboratory is permitted under a Compliance Agreement
(No. 07-22, 7/19/94) with the US Department of Agriculture (CFR330.300-302) to receive and
analyze soil samples from foreign sources or regulated areas within the country. Samples
must be shipped directly to CytoCulture with a USDA label indicating the restricted entry
of the soil samples for testing purposes. CytoCulture can fax or mail these labels to lab
clients wishing to ship foreign samples. A standard operating procedure, approved by the
USDA, is used to autoclave all quarantined samples after testing and prior to disposal.
Please Contact us for a
customize chain of custody form for your laboratory. For a general chain of custody form
in MS Word Format, follow this link: Chain of Custody.
If you do not have MS Word or a compatible word
processor, MS Word viewer can be downloaded for
printing and viewing this document.